Survey Protocols & Survey Forms


Leaf sampling for MLN surveillance

Survey Protocols and Survey Forms

Standardised survey and sampling protocols for MLN have been developed by CIMMYT and partners. Field surveys have been designed to collect accurate data on the presence of MCMV throughout major maize growing areas – both farmer fields and seed production fields. Recommended field survey methodology focuses on in-situ leaf sampling, coupled to Agristrip testing with confirmation through ELISA testing. Seed sampling is coupled to ELISA testing. All surveys, both field and seed are geo-referenced. The aim is to collect standardised information on the distribution of MLN to serve as a regional resource for planning and control.

Download the MLN Survey and Sampling Protocol


Survey Forms

Standardised survey forms have been developed by CIMMYT and national partners for MLN field surveys and also seed collection surveys. Both surveys have been implemented in electronic format using ODK (Electronic survey solutions for Android devices are available for download), but paper versions of survey forms can be downloaded from this page. Paper survey forms should be used in combination with a GPS. Guidelines for use of GPS are also included for download:

Download the MLN Field Survey Form

Download the MLN Seed Survey Form

Download GPS Guidelines

Data submission forms:

Download the MLN Field data sheet

Download the MLN Seed data sheet


For more information about surveys and sampling, please contact:


Dr. Suresh, L.M.  | Maize Pathologist – Sub Saharan Africa, Global Maize Program &

Manager – MLN Screening facility

International Maize and Wheat Improvement Center (CIMMYT)

World Agroforestry Centre (ICRAF), United Nations Avenue, Gigiri. P.O.Box 1041–00621, Nairobi, Kenya

tel: +254 (20) 7224069  skype: suresh.lm   cel: +254 702392664 Tweeter : _lmsuresh


Dr. Dave Hodson

International Maize and Wheat Improvement Center (CIMMYT)

ILRI Gurd Shola Campus, P.O. Box 5689, Addis Ababa, Ethiopia

Email: tel: +251 (0) 116172302  Skype: hodsomex